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KMID : 0385019940100020185
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Korean Journal of Laboratory Animal Science
1994 Volume.10 No. 2 p.185 ~ p.196
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Transgenic Mice Carrying Human Gastrin/SV 40 Large T Antigen Fusion Genes
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김선정/Kim, Sun Jung
이철상/이경광/한용만/유욱준/강부현/백광희/Lee, Chul Sang/Lee, Kyung Kwang/Han, Yong Mahn/Yoo, Ook Joon/Kang, Boo Hyon/Baek, Kwang Hee
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Abstract
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Tissue-specific tumorigenesis can be induced in transgenic mice by the directed expression of SV40 large T antigen. In an attempt to induce the tumors or cancers in stomach tissues, nine transgenic mice were genrated that harbored a chimeric gene composed of the SV40 large T antigen gene fused to the exon II of a human gastrin gene including 5´ and 3´ flanking sequences- It was represented through germ-line transmission that three transgene mice were mosaic. The transgene was expressed in all tissues examined by reverse transcription DNA polymerase chain reaction (RT-PCR) whereas endogenous mouse gastrin transcripts were detected only in both stomach and brain tissues of the transgenic mouse. The antral region of transgenic mice (TEX-3 line) exhibited abnormal state in appearance although stomach sections of transgenic mice have yet to show any histological or pathological changes.
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